Expression Database

GUDMAP:22130

Gene:	Htr3a, 5-hydroxytryptamine (serotonin) receptor 3A
Stage:	Theiler Stage 22
Tissue:
Species:	Mus musculus
Assay Type:	TG mouse marker strain

Images

(A) Mid-sagital image captured from direct fluorescence of sectioned urogenital tract from 14.5dpc fetal Htr3a-EGFP transgenic mouse. EGFP fluorescence is dorsal root ganglia (drg), pelvic ganglia (pg), and peripheral nerve fibers running between these ganglia. Non-neuronal expression is also observed in the distal tip of the genital tubercle (B) Sagital in situ sectional image from Eurexpress.org (image euxassay_008385_ 12) with contrast enhanced to reveal transcription of the Htr3a gene in pelvic ganglia and dorsal root ganglia. Blood vessel flanking the bladder (bv) and genital tubercle (gt).

Results Notes

Submitter finding: Expression of Htr3a-EGFP was detected in 14.5 dpc fetal mice in anatomical locations consistent with developing peripheral nervous system elements. Htr3a-EGFP BAC transgene expression was comparable to whole mount in situ hybridization for Tyrosine hydroxylase.

Mouse marker strain entries are unique GUDMAP entries that can contain data for multiple probes at multiple stages of development (more info).

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	K. Elaine Ritter, E Michelle Southard-Smith
Submitted By:	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Batch ID:	568
Submission ID:	22130

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

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Specimen

Stage:

TS22

Other Staging System:

14.5 dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type
Notes:		Mouse strain was obtained from Mutant Mouse Regional Resource Center (Stock Number: 000273-UNC-RESUS). Animals were maintained by breeding on a Swiss Webster outbred background.

Sex:

unknown

Specimen Preparation:

mouse marker strain

Fixation Method:

unspecified

Experiment Notes:

Transgene Visualisation

Reporter:	GFP
Direct method for visualising reporter:	Fluorescence

Acknowledgements

A comprehensive PDF containing allele genotyping details and characterisation can be downloaded from the GUDMAP mouse strains page (http://www.gudmap.org/Resources/MouseStrains/Mouse_Strains_Summary.html). GUDMAP:21130 entry compiled from this PDF by GUDMAP Editorial Office.