Expression Database

GUDMAP:22048

Gene:	Htr3a, 5-hydroxytryptamine (serotonin) receptor 3A
Stage:	Theiler Stage 22
Tissue:	dorsal root ganglion
Species:	Mus musculus
Assay Type:	TG section

Images

	Red channel shows Trpv1 expression; green channel shows Htr3a-EGFP expression.
	Green channel shows Htr3a-EGFP expression.
	Red channel shows Trpv1 expression.

Results Notes

Expression of Htr3a-EGFP transgene and Trpv1 is noted in T13 dorsal root ganglion (EMAPA:25186). EGFP expression is present, strong, and visible in single neuronal cell bodies of the ganglion. Trpv1 expression is present, moderate to weak, and spotted throughout the neuronal cell bodies of the ganglion.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	K. Elaine Ritter
Submitted By:	Elaine Ritter, Vanderbilt University, Nashville, TN, USA, elaine.ritter@vanderbilt.edu
Batch ID:	554
Submission ID:	22048

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

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Specimen

Stage:

TS22

Other Staging System:

14 dpc

Tissue:

dorsal root ganglion

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	Neutral buffered formalin
	Embedding:	Cryosection

Experiment Notes:

20uM sagittal cryo-section of 14dpc Htr3a-EGFP embryo. Camera settings for images 1, 2 & 3: LSM 510 Meta Confocal Microscope; Objective: Plan-Apochromat 20x/0.75; Imaged Area Size: 450uM x 450uM x 8uM; Scan Mode: Plane, multi-track, 8 bit; Laser Power for Image 1: 488nm - 11%, 633nm - 36%; Laser Power for Image 2: 488nm - 11%; Laser Power for Image3: 633nm - 36%; Pinhole Size: 82uM both channels.

Transgene Visualisation

Reporter:	EGFP
Direct method for visualising reporter:	transgene fluorescence

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:21914	same section