Expression Database

GUDMAP:22047

Gene:	Htr3a, 5-hydroxytryptamine (serotonin) receptor 3A
Stage:	Theiler Stage 22
Tissue:	dorsal root ganglion
Species:	Mus musculus
Assay Type:	TG section

Images

	Blue channel shows Tac1 expression; green channel shows Htr3a-EGFP expression.
	Green channel shows Htr3a-EGFP expression.
	Blue channel shows Tac1 expression.

Results Notes

Expression of Htr3a-EGFP transgene and Tac1 (Substance P) in S1 dorsal root ganglion (EMAPA:25170). EGFP is present, strong, and visible in single neuronal cell bodies and axons. Tac1 (Substance P) is present, moderate to weak, and spotted. Both are expressed in neuronal cell bodies and axons.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	K. Elaine Ritter
Submitted By:	Elaine Ritter, Vanderbilt University, Nashville, TN, USA, elaine.ritter@vanderbilt.edu
Batch ID:	554
Submission ID:	22047

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

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Specimen

Stage:

TS22

Other Staging System:

14 dpc

Tissue:

dorsal root ganglion

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Cryosection

Experiment Notes:

20uM sagittal cryo-sections were mounted on 3-APES-treated slides and stained for Substance P via IHC. Co-expression of Htr3a-EGFP transgene and Substance P stain was noted in dorsal root ganglia. Camera settings for images 1, 2 & 3: LSM 510 Meta Confocal Microscope; Objective: Plan-Apochromat 20x/0.75; Imaged Area Size: 450uM x 450uM Scan Mode: Plane, multi-track, 8 bit; Laser Power for Image 1: 488nm - 9%, 633nm - 35%; Laser Power for Image 2: 488nm – 9%; Laser Power for Image 3: 633nm - 35%; Pin hole Size: 88uM both channels.

Transgene Visualisation

Reporter:	EGFP
Direct method for visualising reporter:	transgene fluorescence

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:21913	same section