Expression Database

GUDMAP:22045

Gene:	Htr3a, 5-hydroxytryptamine (serotonin) receptor 3A
Stage:	Theiler Stage 22
Tissue:	dorsal root ganglion
Species:	Mus musculus
Assay Type:	TG section

Images

	Red channel (Cy3) shows P2rx3 expression; green channel shows Htr3a-EGFP expression. Magnification: x20.
	Green channel shows Htr3a-EGFP expression. Magnification: x20.
	Red channel shows P2rx3 expression. Magnification: x20.

Results Notes

Expression of Htr3a-EGFP transgene and P2rx3 is observed in the dorsal root ganglion (EMAPA:16668). EGFP expression is present, strong, and visible in single neuronal cell bodies of the ganglion, as well as axons.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	Dennis P. Buehler
Submitted By:	Dennis P Buehler, Vanderbilt University, Nashville, TN, USA, dennis.p.buehler@vanderbilt.edu
Batch ID:	554
Submission ID:	22045

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Specimen

Stage:

TS22

Other Staging System:

14.5 dpc

Tissue:

dorsal root ganglion

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	Neutral buffered formalin
	Embedding:	Cryosection

Experiment Notes:

Camera settings for images 1, 2 & 3: LSM 510 Meta Confocal Microscope; Objective: Plan-Apochromat 20x/0.75; Laser Power for Image 1: 488nm-8%, 543nm-40%; Laser Power for Image 2: 488nm-8%; Laser Power for Image 3: 543nm-40%; Filters: BP 505-550 (EGFP), BP 560-615 (Cy3); Pinhole 120um; Tiled image.

Transgene Visualisation

Reporter:	EGFP
Direct method for visualising reporter:	transgene fluorescence

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:21852	same section