Expression Database

GUDMAP:21896

Gene:	Crim1, cysteine rich transmembrane BMP regulator 1 (chordin like)
Stage:	Theiler Stage 23
Tissue:	urinary system
Species:	Mus musculus
Assay Type:	TG mouse marker strain

Images

	Cre-dependent ?-gal activity in Crim1GCE/+; R26RlacZ/+ UGS samples. Two doses of Tamoxifen (lower four panels: 2mg/40g) results in a small number of X-gal positive cells in the adrenal glands, kidney and the male and female reproductive systems in Crim1GCE/+; R26RlacZ/+ embryos at 15.5dpc.
	Cre-dependent ?-gal activity in Crim1GCE/+; R26RlacZ/+ 15.5dpc UGS samples. A single dose of Tamoxifen at 13.5dpc results in a small number of X-gal positive cells in the microvasculature of Crim1GCE/+; R26RlacZ/+ embryos at 15.5dpc. With two doses of Tamoxifen, given at 10.5 and 12.5dpc, the number of positive cells increased in the Adrenal gland and renal blood vessels.
	ß-gal positive cells are detected in a subset of PDGFR-b positive endothelial cells lining microvasculature in Crim 1GCE/+; R26RlacZ/+ embryos at 15.5dpc. Antibodies: rat-anti-PDGFR-b/ rabbit-anti-ß-gal.
	ß-gal positive cells are detected in a percentage of Smooth muscle actin positive arterioles in Crim 1GCE/+; R26RlacZ/+ embryos at 15.5dpc. Antibodies: mouse-anti-SMA/ rabbit-anti-ß-gal.

Results Notes

Submitter findings: Analysis confirms the expression of Crim1 driven eGFPCreERT2 in a subset of cells in renal vasculature at 15.5 dpc. Native GFP expression was not detectable in our samples and could not be visualized by immunohistochemistry. Cre dependent R26RLacZ expression was observed in a subset of PDGFR-b positive endothelial cells following Tamoxifen treatment.

Mouse marker strain entries are unique GUDMAP entries that can contain data for multiple probes at multiple stages of development (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Keck School of Medicine, Los Angeles, CA, USA, amcmahon@med.usc.edu
Contributors:	Andrew P McMahon, M Todd Valerius, Jinjin Guo, Jill McMahon, Pumin Zhang
Submitted By:	Jill McMahon, Keck School of Medicine, Los Angeles, CA, USA
Batch ID:	555
Submission ID:	21896

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Specimen

Stage:

TS23

Other Staging System:

15.5 dpc

Tissue:

urinary system

Strain:

mixed background

Genotype:

Non-wild type Allele:
Gene		Crim1
MGI ID		MGI:5317218
MGI Symbol or lab name		Crim1^{tm1(GFP/cre/ERT2)Pzg}
Type		Targeted, (Recombinase\|Reporter\|Inducible)
Allele First Chromatid		Crim1^{tm1(GFP/cre/ERT2)Pzg}
Allele Second Chromatid		wild type
Notes:		Mouse strain is available from the Jackson Lab, see here: http://jaxmice.jax.org/strain/017495.html

Sex:

both

Specimen Preparation:

mouse marker strain

Fixation Method:

unspecified

Experiment Notes:

Immunohistochemistry was performed to examine if the eGFPCreERT2 allele was expressed in the expected Crim1 domain. To test for Cre function,15.5dpc Crim1GCE/+; R26RlacZ/+ and R26RlacZ/+ embryos from Tamoxifen and corn oil injected mice were assayed. Whole UGS samples were fixed in 4% paraformaldehyde at 4ºC 2 hours, washed 3 times in PBS, equilibrated in 30% sucrose overnight, embedded in OCT and flash frozen on dry ice. The samples were sectioned at 16μm and probed with chicken-anti- GFP/ rat-anti-Pecam CD31/ rabbit-anti-ß-Gal and rabbit-anti-ß-gal/chicken-anti-GFP/ Mouse anti-Smooth muscle Actin.

Transgene Visualisation

Reporter:	GFP
Direct method for visualising reporter:	Fluorescence

Acknowledgements

Comprehensive PDFs containing allele verification (provided by Zhang lab) and allele characterisation (provided by McMahon lab) can be downloaded from the GUDMAP mouse strains page (http://www.gudmap.org/Resources/MouseStrains/Mouse_Strains_Summary.html). GUDMAP:21896 entry compiled from these PDFs by GUDMAP Editorial Office.