Expression Database

GUDMAP:21892

Gene:	Foxl2, forkhead box L2
Stage:	Theiler Stage 23
Tissue:	urinary system
Species:	Mus musculus
Assay Type:	TG mouse marker strain

Images

	Cre-dependent ?-gal activity in Foxl2GCE/+; R26RlacZ/+ UGS samples. A single dose of Tamoxifen (1mg/40g) results in a small number of X-gal positive cells in granulosa cell precursors located in the ovarian medulla of Foxl2GCE/+; R26RlacZ/+ embryos at 15.5dpc.
	Cre-dependent ?-gal activity in Foxl2GCE/+; R26RlacZ/+ UGS samples. A single dose of Tamoxifen (1mg/40g) results in a small number of X-gal positive cells in the medulla of the ovaries of Foxl2GCE/+; R26RlacZ/+ embryos at 15.5dpc.
	GFP is detected in cells adjacent to E-Cadherin positive germ cells in the medulla of the ovary in Foxl2GCE/+; R26RlacZ/+ embryos at 14.5dpc. Antibodies: chicken-anti-GFP/ rat-anti-E-Cadherin / rabbit-anti-Laminin.
	GFP is detected in granulosa precursor cells distinct from E-Cadherin positive germ cells, in the ovary of Foxl2GCE/+; R26RlacZ/+ embryos at 14.5dpc. Antibodies: chicken-anti-GFP/ rat-anti-E-Cadherin.
	Co-localization of ?-gal positive cells and GFP positive cells following Tamoxifen induction of Foxl2 driven GCE. Co-localization is observed in putative granulosa precursor cells in the center of the medulla in Foxl2GCE/+; R26RlacZ/+ ovaries at 14.5dpc. Antibodies: chicken-anti-GFP / rabbit-anti-?-gal / rat-anti-E-Cadherin.

Results Notes

Submitter findings: Analysis confirms the expression of Foxl2 driven eGFPCreERT2 in a small population of cells located in the medulla of the ovary at 14.5-15.5 dpc. The labeled cells are likely granulosa cell precursors. Native GFP expression was not detectable in our samples but could be visualized by immunohistochemistry. Cre dependent R26RLacZ expression was observed in a subset of GFP positive cells following Tamoxifen treatment.

Mouse marker strain entries are unique GUDMAP entries that can contain data for multiple probes at multiple stages of development (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Keck School of Medicine, Los Angeles, CA, USA, amcmahon@med.usc.edu
Contributors:	Andrew P McMahon, M Todd Valerius, Jinjin Guo, Jill McMahon
Submitted By:	Jill McMahon, Keck School of Medicine, Los Angeles, CA, USA
Batch ID:	555
Submission ID:	21892

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Specimen

Stage:

TS23

Other Staging System:

15.5 dpc

Tissue:

urinary system

Strain:

mixed background

Genotype:

Non-wild type Allele:
Gene		Foxl2
MGI ID		MGI:4940388
MGI Symbol or lab name		Foxl2^{tm1(GFP/cre/ERT2)Pzg}
Type		Targeted, (Reporter\|Inducible\|Recombinase)
Allele First Chromatid		Foxl2^{tm1(GFP/cre/ERT2)Pzg}
Allele Second Chromatid		wild type
Notes:		Mouse strain is available from the Jackson Lab, see here: http://jaxmice.jax.org/strain/015854.html

Sex:

both

Specimen Preparation:

mouse marker strain

Fixation Method:

unspecified

Experiment Notes:

Transgene Visualisation

Reporter:	GFP
Direct method for visualising reporter:	Fluorescence

Acknowledgements

Comprehensive PDFs containing allele verification (provided by Zhang lab) and allele characterisation (provided by McMahon lab) can be downloaded from the GUDMAP mouse strains page (http://www.gudmap.org/Resources/MouseStrains/Mouse_Strains_Summary.html). GUDMAP:21892 entry compiled from PDFs by GUDMAP Editorial Office.