Expression Database

GUDMAP:21880

Gene:	Entpd5, ectonucleoside triphosphate diphosphohydrolase 5
Stage:	Theiler Stage 23
Tissue:	urinary system
Species:	Mus musculus
Assay Type:	TG mouse marker strain

Images

	Very low levels of endogenous eGFP activity was observed in kidneys of Entpd5G2aCE/+ mice. Robust tamoxifen dependent tdTomato fluorescence was observed in proximal tubule cells. Tamoxifen injection at 17.5dpc and kidney collection at P0. Low power image.
	Very low levels of endogenous eGFP activity was observed in kidneys of Entpd5G2aCE/+ mice. Robust tamoxifen dependent tdTomato fluorescence was observed in proximal tubule cells. Tamoxifen injection at 17.5dpc and kidney collection at P0. Low power image.
	Cre-dependent ?-gal activity was detected within the proximal tubules in P0 kidneys (Entpd5G2aCE/+; R26RlacZ/+) following tamoxifen injection at 17.5dpc.
	Tamoxifen dependent Cre activity in Entpd5G2aCE/+; R26RtdTomato/+ P0 kidneys colocalized with eGFP expressing cells. TdTomato fluorescence was observed in a percentage of eGFP+, proximal tubules within the P0 kidney following tamoxifen injection at 17.5dpc.
	TdTomato fluorescence was detected in LTL+ proximal tubule cells in Entpd5G2aCE/+; R26RtdTomato/+ P0 kidneys upon tamoxifen-mediated Cre recombination. Co-localization of LTL+ and tdTomato+ cells in proximal tubules of the P0 kidney following tamoxifen injection at 17.5dpc.
	Cre dependent tdTomato expression was detected in proximal tubule cells following tamoxifen induction in Entpd5G2aCE/+; R26RtdTomato/+ adult kidneys. LTL+ and tdTomato+ proximal tubules were observed in the adult kidney following tamoxifen injection 3 times prior to collection: d1, d3, d5 collected at d7.

Results Notes

Submitter findings: Analysis confirms the expression of eGFP and activity of CreErt2 under the regulation of Entpd5 in proximal tubule cells of the mouse kidney at 19.5 days post coitum. Weak endogenous eGFP expression within the proximal tubule was confirmed by immunohistochemistry. Upon induction with tamoxifen, Cre dependent R26R LacZ and tdTomato expression was observed in Lotus tetragonolobus lectin (LTL) positive proximal tubule cells in the embryonic and adult kidney.

Mouse marker strain entries are unique GUDMAP entries that can contain data for multiple probes at multiple stages of development (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Keck School of Medicine, Los Angeles, CA, USA, amcmahon@med.usc.edu
Contributors:	Andrew P McMahon, Jinjin Guo, Jill McMahon
Submitted By:	Jill McMahon, Keck School of Medicine, Los Angeles, CA, USA
Batch ID:	555
Submission ID:	21880

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

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Specimen

Stage:

TS23

Other Staging System:

15.5 dpc

Tissue:

urinary system

Strain:

mixed background

Genotype:

Non-wild type Allele:
Gene		Entpd5
MGI ID		MGI:5565343
MGI Symbol or lab name		Entpd5^{tm1(GFP,cre/ERT2)Amc}
Type		Targeted, (Reporter\|RMCE-ready
Allele First Chromatid		Entpd5^{tm1(GFP,cre/ERT2)Amc}
Allele Second Chromatid		wild type
Notes:		Mouse strain is available from the Jackson Lab, see here: http://jaxmice.jax.org/strain/025208.html

Sex:

both

Specimen Preparation:

mouse marker strain

Fixation Method:

unspecified

Experiment Notes:

Entpd5 G2aCE /+ male chimeras were mated to R26RlacZ/lacZ and R26R tdTomato/tdTomato females to generate Entpd5G2aCE /+; R26RlacZ/+ or R26RtdTomato/+ embryos/pups. In order to activate ßgalactosidase (β-gal) or tdTomato reporter expression, pregnant females were injected intraperitoneally at 17.5dpc with tamoxifen in corn oil (1X 2mg to 40g body weight) and the kidneys assayed at P0. A control group was injected with the same volume of corn oil. Tamoxifen dependent Cre activity was detected in proximal tubule cells in Entpd5 G2aCE /+; R26RlacZ/+ and Entpd5 G2aCE /+; R26R tdTomato/+ samples.

Transgene Visualisation

Reporter:	GFP
Direct method for visualising reporter:	Fluorescence

Acknowledgements

A comprehensive PDF containing allele verification and characterisation can be downloaded from the GUDMAP mouse strains page (http://www.gudmap.org/Resources/MouseStrains/Mouse_Strains_Summary.html). GUDMAP:21880 entry compiled from this PDF by GUDMAP Editorial Office.