Expression Database

GUDMAP:21361

Gene:	Chat, choline acetyltransferase
Stage:	Theiler Stage 22
Tissue:	bladder,pelvic ganglion,genital tubercle of male
Species:	Mus musculus
Assay Type:	TG section

Images

	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage.Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red. Includes pelvic ganglion.
	Montage. Includes pelvic ganglion.
	Transverse section. Montage. Dorsal up. Double fluorescent immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red. Genital tubercle of male.
	Transverse section. Montage. Greyscale. Dorsal up. Genital tubercle of male.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red. Includes pelvic ganglion.
	Montage. Dorsal up. Includes pelvic ganglion.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red. Includes pelvic ganglion.
	Montage. Dorsal up. Includes pelvic ganglion.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.
	Montage. Dorsal up. Double fluorescence immunohistochemistry, ChAT (GFP) = green and PGP 9.5 = red.
	Montage. Dorsal up.

Submitters

Principal Investigator(s):	Janet Keast, University of Melbourne, Melbourne, Australia, janet.keast@unimelb.edu.au
Contributors:	Adam Wallace, Janet Keast
Submitted By:	Adam Wallace, University of Melbourne, Melbourne, Australia, a.wallace@unimelb.edu.au
Batch ID:	377
Submission ID:	JK_080413_163

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Specimen

Stage:

TS22

Other Staging System:

14.5 dpc

Tissue:

bladder,pelvic ganglion,genital tubercle of male

Strain:

unspecified

Genotype:

Non-wild type Allele 1:
Gene		Chat
MGI ID		MGI:3689420
MGI Symbol or lab name		Chat^tm1(cre)Lowl
Type		Targeted (knock-in)
Allele First Chromatid		Chat^tm1(cre)Lowl
Allele Second Chromatid		wild type

Non-wild type Allele 2:
Gene		Gt(ROSA)26Sor
MGI ID		MGI:2449038
MGI Symbol or lab name		Gt(ROSA)26Sor^tm1(EYFP)Cos
Type		Targeted (Reporter)
Allele First Chromatid		Gt(ROSA)26Sor^tm1(EYFP)Cos
Allele Second Chromatid		wild type

Sex:

male

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Cryosection

Experiment Notes:

Transverse section. Section co-stained with rabbit anti-human PGP9.5 antibody (Dilution = 1:2000, Cedarlane Laboratories Ltd #CL95101). Secondary antibody donkey anti-rabbit Alexa 594 (Dilution = 1:1000, Molecular Probes, Invitrogen).

Transgene Visualisation

Reporter:	GFP
Direct method for visualising reporter:	anti-GFP immunohistochemitry

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:21372	same section