Expression Database

GUDMAP:8205

Gene:	Aqp2, aquaporin 2
Stage:	Theiler Stage 28
Tissue:	metanephros
Species:	Mus musculus
Assay Type:	IHC section

Images

	Metanephros with proximal ureter.
	Cortex.
	Outer stripe of outer medulla.
	Inner stripe of outer medulla.
	Inner medulla.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Contributors:	Kylie Georgas, Bree Rumballe, Han Sheng Chiu
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Batch ID:	60
Submission ID:	ml_280507_6226

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: Aqp2

Name: aquaporin 2 (MGI:1096865)

Name:

anti-AQP2

Accession ID:

MGI:4848137 (maprobe:4431)

Epitope (Uniprot ID):

P34080

Amino-terminus (start):

254

Carboxy-terminus (end):

271

Supplier:

Chemicon

Catalogue Number:

AB3274

Lot Number:

Type:

Polyclonal

Host:

rabbit

Purification Method:

affinity purified

Immunoglobulin Isotype:

Variant Detected:

Species Specificity:

human, rat

Curator Notes:

Current supplier: Merck Millipore cat# AB3274.

Detection System:

Direct Label:

Secondary Antibody: anti-rabbit IgG (H+L), HRP conjugated antibody (Promega W4011)

Signal Detection Method: DAB (DAB Peroxidase Substrate Kit, Vector Laboratories, SK 4100) (brown-orange)

Detection Notes:

Dilution:

Notes:

Antigen: highly purified peptide aa254 to 271 rat or mouse Aquaporin2 SwissProt accession number P34080. Rat and human are identical for this epitope. Human cross reactivity confirmed. Sheep and human identical for 17/18 aa.

Lab Antibody ID:

anti-AQP2

Specimen

Stage:

TS28

Other Staging System:

Adult sexually mature

Tissue:

metanephros

Strain:

CD1

Genotype:

wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Paraffin

Experiment Notes:

8 metanephros 7um sections. Immunohistochemistry with anti-aquaporin2 antibody from Chemicon Catalogue number AB3274 visualised with DAB which produces orange signal. Experiment: 060606 Imaging: Batchscan020706_Folder060709_4.vsi&Folder060705_15.vsi. Aquaporin 2 protein is expressed in the apical membrane of collecting duct principal cells. Reviewed in King and Yasui 2002 TRENDS in Endocrinology & Metabolism Vol.13 No.8.

Linked Publications

Kylie Georgas, Bree Rumballe, Lorine Wilkinson, Han Sheng Chiu, Emmanuelle Lesieur, Thierry Gilbert, Melissa H. Little
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney
Histochem Cell Biol, 2008, 130(5):927-42
PubMed: 18618131