Expression Database

GUDMAP:8204

Gene:	Aqp1, aquaporin 1
Stage:	Theiler Stage 28
Tissue:	metanephros,ureter
Species:	Mus musculus
Assay Type:	IHC section

Images

	Metanephros with proximal ureter.
	Cortex. Aqp1 protein detected in segment 2 proximal tubules and in red blood cells and cortical capillaries (see far right).
	Outer stripe of outer medulla.
	Inner stripe of outer medulla. Note vascular bundle.
	Inner stripe of outer medulla.
	Inner medulla.
	Cortex. Aqp1 protein detected in cortical capillaries and red blood cells. Protein absent from arterial system.
	Inner medulla and pelvis. Protein detected in pelvic smooth muscle.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Contributors:	Kylie Georgas, Bree Rumballe, Han Sheng Chiu
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Batch ID:	60
Submission ID:	ml_280507_6225

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: Aqp1

Name: aquaporin 1 (MGI:103201)

Name:

anti-AQP1

Accession ID:

MGI:4848131 (maprobe:4429)

Epitope (Uniprot ID):

P29975

Supplier:

Chemicon

Catalogue Number:

AB3065

Lot Number:

Type:

Polyclonal

Host:

rabbit

Purification Method:

affinity purified

Immunoglobulin Isotype:

Variant Detected:

Species Specificity:

rat, mouse, human

Curator Notes:

Current supplier details: Merck Millipore cat# AB2219.

Detection System:

Direct Label:

Secondary Antibody: anti-rabbit IgG (H+L), HRP conjugated antibody (Promega W4011)

Signal Detection Method: DAB (DAB Peroxidase Substrate Kit, Vector Laboratories, SK 4100) (brown-orange)

Detection Notes:

Dilution:

Notes:

Antigen: 19aa synthetic peptide from cytosolic carboxyl terminal domain of AQP1_RAT. Peptide contains most of the epitopes recognised by polyclonal antibodies against the whole protein.

Lab Antibody ID:

anti-AQP1

Specimen

Stage:

TS28

Other Staging System:

Adult sexually mature

Tissue:

metanephros,ureter

Strain:

CD1

Genotype:

wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Paraffin

Experiment Notes:

8 metanephros 7um sections. Immunohistochemistry with anti-aquaporin1 antibody from Chemicon Catalogue number AB3065 visualised with DAB which produces orange signal. Experiment: 290606 Imaging: Batchscan020706_Folder060706_4&6.vsi&Folder060707_1&2.vsi. Published data from rat shows Aqp1 localised to thin descending limb of loop of Henle and descending vasa recta and labeling of cortical lymphatic vessels. Nielsen et al 1995 Am J Physiol 268 p.F1023-F1037.

Linked Publications

Kylie Georgas, Bree Rumballe, Lorine Wilkinson, Han Sheng Chiu, Emmanuelle Lesieur, Thierry Gilbert, Melissa H. Little
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney
Histochem Cell Biol, 2008, 130(5):927-42
PubMed: 18618131

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:10912	control
GUDMAP:10915	control
GUDMAP:10919	control