Expression Database

GUDMAP:8202

Gene:	Aqp1, aquaporin 1
Stage:	Theiler Stage 23
Tissue:	metanephros,ureter
Species:	Mus musculus
Assay Type:	IHC section

Images

	Metanephros.
	Nephrogenic zone and cortex. Note Aqp1 protein in proximal tubules and glomerular capillary system
	Nephrogenic zone and cortex. Note Aqp1 protein in invading endothelial and red blood cells in the cleft of the S-shaped body and the glomerular capillary system of the maturing renal corpuscle.
	Cortex. Note Aqp1 protein in proximal tubules and capillaries.
	Cortex. Aqp1 protein in artery and red blood cells and juxtaglomerular arteriole.
	Cortex. Note Aqp1 protein in juxtaglomerular arteriole of maturing renal corpuscle.
	Cortex and medulla. Note strong Aqp1 protein signal in capillaries extending into the medulla.
	Cortex and medulla. Note Aqp1 signal in early proximal tubule and a subset of the loop of Henle which may represent early descending limb.
	Pelvis medulla and edge of cortex. Note Aqp1 protein in early proximal tubule and cortical and medullary capillaries and pelvic smooth muscle. Aqp1 protein is absent from the loop of Henle
	Ureter. Aqp1 protein in ureteral mesenchyme.
	Global view.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Contributors:	Kylie Georgas, Bree Rumballe, Han Sheng Chiu
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Batch ID:	60
Submission ID:	ml_280507_6223

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: Aqp1

Name: aquaporin 1 (MGI:103201)

Name:

anti-AQP1

Accession ID:

MGI:4848131 (maprobe:4429)

Epitope (Uniprot ID):

P29975

Supplier:

Chemicon

Catalogue Number:

AB3065

Lot Number:

Type:

Polyclonal

Host:

rabbit

Purification Method:

affinity purified

Immunoglobulin Isotype:

Variant Detected:

Species Specificity:

rat, mouse, human

Curator Notes:

Current supplier details: Merck Millipore cat# AB2219.

Detection System:

Direct Label:

Secondary Antibody: anti-rabbit IgG (H+L), HRP conjugated antibody (Promega W4011)

Signal Detection Method: DAB (DAB Peroxidase Substrate Kit, Vector Laboratories, SK 4100) (brown-orange)

Detection Notes:

Dilution:

Notes:

Antigen: 19aa synthetic peptide from cytosolic carboxyl terminal domain of AQP1_RAT. Peptide contains most of the epitopes recognised by polyclonal antibodies against the whole protein.

Lab Antibody ID:

anti-AQP1

Specimen

Stage:

TS23

Other Staging System:

15.5 dpc

Tissue:

metanephros,ureter

Strain:

CD1

Genotype:

wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Paraffin

Experiment Notes:

14 metanephros 7um sections including proximal ureter. Immunohistochemistry with anti-Aquaporin1 antibody from Chemicon Catalogue number AB3065 visualised with DAB which produces orange signal. Experiment: 060606 Imaging: Batchscan020706_Folder060705_5&8.vsi

Linked Publications

Kylie Georgas, Bree Rumballe, Lorine Wilkinson, Han Sheng Chiu, Emmanuelle Lesieur, Thierry Gilbert, Melissa H. Little
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney
Histochem Cell Biol, 2008, 130(5):927-42
PubMed: 18618131

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:10913	control
GUDMAP:10916	control
GUDMAP:10917	control