Expression Database

GUDMAP:23040
Gene: ACTA1, actin, alpha 1, skeletal muscle
Stage: Fetal Stage - 14th week post-fertilization
Tissue: bladder
Species: Homo sapiens
Assay Type: IHC section
Images
Whole distal neck. Very low level of expression may be present in the outer layers. Site of transition to urethra not determined.
Whole distal neck + DAPI. ACTA1 = green, DAPI = blue. Site of transition to urethra not determined.
Distal neck wall. Site of transition to urethra not determined.
Distal neck wall + DAPI. ACTA1 = green, DAPI = blue. Site of transition to urethra not determined.
Results Notes
All tissue examined showed blood cell autofluorescence that is not specific to gene expression.
Submitters
Contributors: Keast Lab
Submitted By: Keast Lab
Batch ID: 579
Submission ID: 23040
Expression Mapping

Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
   
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
   
Nerve Density:

Relative to Total:
High
Medium
Low
   
Relative to P0/Adult:
Increase, large
Increase, small
Decrease, large
Decrease, small
   
Contains note
View annotated components as a list Show annotation under groups information
Antibody
Protein:
Symbol: ACTA1
Name: actin, alpha 1, skeletal muscle (NCBI ID: 58)
Name: Skeletal Muscle Actin Antibody
Accession ID: No MGI ID (maprobe:9092)
Epitope (Uniprot ID): unknown
   
Supplier: Thermo Scientific
Catalogue Number: MA1-37021
Lot Number:
   
Type: Monoclonal
Host:
Purification Method: Protein A/G
   
Immunoglobulin Isotype: IgM
Variant Detected: 5C5.F8.C7
Species Specificity: human, rat, rabbit, guinea pig
Curator Notes:
Detection System:
Direct Label:
Secondary Antibody: Donkey anti-mouse Alexa Fluor 594 (Molecular Probes, ThermoFisher Scientific, cat# A-21203)
Signal Detection Method: Alexa Fluor 594
Detection Notes: Secondary antibody dilution 1:500
   
Dilution:
Notes:
Lab Antibody ID:
Specimen
Stage: Fetal 14wk
Tissue: bladder
Strain:
Genotype:
Sex: female
Specimen Preparation:
section
Fixation Method: 4% paraformaldehyde
Embedding: Cryosection
Experiment Notes:
De-identified tissue was obtained following informed consent.

 

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