Expression Database

Tissue Summaries

Expression Database

GUDMAP:22928

Gene:	ACTA1, actin, alpha 1, skeletal muscle
Stage:	Fetal Stage - 18th week post-fertilization
Tissue:	bladder,bladder-urethra transition zone
Species:	Homo sapiens
Assay Type:	IHC section

Images

	Whole distal neck. Moderate level of expression in the outer layers. Possible transition to urethra.
	Whole distal neck + DAPI. ACTA1 = green, DAPI = blue. Moderate level of expression in the outer layers. Possible transition to urethra.
	Distal neck wall. Moderate level of expression in the outer layers. Possible transition to urethra.
	Distal neck wall + DAPI. ACTA1 = green, DAPI = blue. Possible transition to urethra.

Results Notes

All tissue examined showed blood cell autofluorescence that is not specific to gene expression.

Submitters

Contributors:	Keast Lab
Submitted By:	Keast Lab
Batch ID:	579
Submission ID:	22928

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: ACTA1

Name: actin, alpha 1, skeletal muscle (NCBI ID: 58)

Name:

Skeletal Muscle Actin Antibody

Accession ID:

No MGI ID (maprobe:9092)

Epitope (Uniprot ID):

unknown

Supplier:

Thermo Scientific

Catalogue Number:

MA1-37021

Lot Number:

Type:

Monoclonal

Host:

Purification Method:

Protein A/G

Immunoglobulin Isotype:

IgM

Variant Detected:

5C5.F8.C7

Species Specificity:

human, rat, rabbit, guinea pig

Curator Notes:

Detection System:

Direct Label:

Secondary Antibody: Donkey anti-mouse Alexa Fluor 594 (Molecular Probes, ThermoFisher Scientific, cat# A-21203)

Signal Detection Method: Alexa Fluor 594

Detection Notes: Secondary antibody dilution 1:1000

Dilution:

Notes:

Lab Antibody ID:

Specimen

Stage:

Fetal 18wk

Tissue:

bladder,bladder-urethra transition zone

Strain:

Genotype:

Sex:

female

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Cryosection

Experiment Notes:

De-identified tissue was obtained following informed consent.


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