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Expression Database
GUDMAP:22927
Gene:
ACTA1
, actin, alpha 1, skeletal muscle
Stage:
Fetal Stage - 13th week post-fertilization
Tissue:
bladder,bladder-urethra transition zone
Species:
Homo sapiens
Assay Type:
IHC section
Images
Whole distal neck. Moderate level of expression in the outer layers. Site of transition to urethra not determined.
Whole distal neck + DAPI. ACTA1 = green, DAPI = blue. Moderate level of expression in the outer layers. Site of transition to urethra not determined.
Distal neck wall. Moderate level of expression in the outer layers. Site of transition to urethra not determined.
Distal neck wall + DAPI. ACTA1 = green, DAPI = blue. Moderate level of expression in the outer layers. Site of transition to urethra not determined.
Results Notes
All tissue examined showed blood cell autofluorescence that is not specific to gene expression.
Submitters
Contributors:
Keast Lab
Submitted By:
Keast Lab
Batch ID:
579
Submission ID:
22927
Expression Mapping
Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
Nerve Density:
Relative to Total:
High
Medium
Low
Relative to P0/Adult:
Increase, large
Increase, small
Decrease, large
Decrease, small
Contains note
View annotated components as a list
Show annotation under groups
Antibody
Protein:
Symbol:
ACTA1
Name:
actin, alpha 1, skeletal muscle
(NCBI ID: 58)
Name:
Skeletal Muscle Actin Antibody
Accession ID:
No MGI ID
(maprobe:9092)
Epitope (Uniprot ID):
unknown
Supplier:
Thermo Scientific
Catalogue Number:
MA1-37021
Lot Number:
Type:
Monoclonal
Host:
Purification Method:
Protein A/G
Immunoglobulin Isotype:
IgM
Variant Detected:
5C5.F8.C7
Species Specificity:
human, rat, rabbit, guinea pig
Curator Notes:
Detection System:
Direct Label:
Secondary Antibody:
Donkey anti-mouse Alexa Fluor 647 (Molecular Probes, ThermoFisher Scientific, cat# A-31571)
Signal Detection Method:
Alexa Fluor 647
Detection Notes:
Secondary antibody dilution 1:500
Dilution:
Notes:
Lab Antibody ID:
Specimen
Stage:
Fetal 13wk
Tissue:
bladder,bladder-urethra transition zone
Strain:
Genotype:
Sex:
male
Specimen Preparation:
section
Fixation Method:
4% paraformaldehyde
Embedding:
Cryosection
Experiment Notes:
De-identified tissue was obtained following informed consent.
