Expression Database

GUDMAP:22881
Gene: ACTA2, actin, alpha 2, smooth muscle, aorta
Stage: Fetal Stage - 18th week post-fertilization
Tissue: bladder,ureter
Species: Homo sapiens
Assay Type: IHC section
Images
Whole fundus. High level of expression in detrusor, blood vessels and muscularis mucosae.
Whole fundus + DAPI. ACTA2= green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae.
Fundus wall. High level of expression in detrusor, blood vessels and muscularis mucosae.
Fundus wall + DAPI. ACTA2 = green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae.
Whole proximal neck (dorsal at top). High level of expression in detrusor, blood vessels and muscularis mucosae.
Whole proximal neck (dorsal at top) + DAPI. ACTA2 = green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae.
Proximal neck wall. High level of expression in detrusor, blood vessels and muscularis mucosae.
Proximal neck wall + DAPI. ACTA2 = green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae.
Whole ureter. High level of expression in muscle layer and blood vessels.
Whole ureter + DAPI. ACTA2 = green, DAPI = blue. High level of expression in muscle layer and blood vessels.
Whole distal neck. High level of expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Whole distal neck + DAPI. ACTA2 = green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Distal neck wall. High level of expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Distal neck wall + DAPI. ACTA2 = green, DAPI = blue. High level of expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Results Notes
All tissue examined showed blood cell autofluorescence that is not specific to gene expression.
Submitters
Contributors: Keast Lab
Submitted By: Keast Lab
Batch ID: 579
Submission ID: 22881
Expression Mapping

Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
   
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
   
Nerve Density:

Relative to Total:
High
Medium
Low
   
Relative to P0/Adult:
Increase, large
Increase, small
Decrease, large
Decrease, small
   
Contains note
View annotated components as a list Show annotation under groups information
Antibody
Protein:
Symbol: ACTA2
Name: actin, alpha 2, smooth muscle, aorta (NCBI ID: 59)
Name: anti-Actin, alpha-Smooth Muscle[clone 1A4]
Accession ID: No MGI ID (maprobe:9039)
Epitope (Uniprot ID): unknown
   
Supplier: Sigma
Catalogue Number: A5228
Lot Number:
   
Type: Monoclonal
Host: Hybridoma 1A4
Purification Method: purified immunoglobulin
   
Immunoglobulin Isotype: IgG2a
Variant Detected:
Species Specificity: mouse, rabbit, human, rat, goat, guinea pig, other species
Curator Notes:
Detection System:
Direct Label:
Secondary Antibody: Donkey anti-mouse Alexa Fluor 488 (Molecular Probes, ThermoFisher Scientific, cat# A-21202)
Signal Detection Method: Alexa Fluor 488
Detection Notes: Secondary antibody dilution 1:500
   
Dilution:
Notes:
Lab Antibody ID:
Specimen
Stage: Fetal 18wk
Tissue: bladder,ureter
Strain:
Genotype:
Sex: female
Specimen Preparation:
section
Fixation Method: 4% paraformaldehyde
Embedding: Cryosection
Experiment Notes:
De-identified tissue was obtained following informed consent.

 

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