Expression Database

GUDMAP:22385
Gene: ACTA2, actin, alpha 2, smooth muscle, aorta
Stage: Fetal Stage - 18th week post-fertilization
Tissue: bladder,ureter
Species: Homo sapiens
Assay Type: IHC section
Images
Fundus. Expression in detrusor, blood vessels and muscularis mucosae.
Fundus wall. Expression in detrusor, blood vessels and muscularis mucosae.
Bladder neck, proximal (dorsal at top). Expression in detrusor, blood vessels and muscularis mucosae. Site of ureter entry at top left and right of the image also shows expression in the smooth muscle.
Bladder neck, trigone. Expression in detrusor, blood vessels and muscularis mucosae.
Bladder neck, ventral. Expression in detrusor, blood vessels and muscularis mucosae.
Whole ureter (dorsal at top). Expression in ureter wall and blood vessels.
Ureter wall. Expression in ureter wall and blood vessels.
Bladder neck, distal (dorsal at top). Expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Bladder neck, distal - dorsal wall. Expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Bladder neck, distal - ventral wall. Expression in detrusor, blood vessels and muscularis mucosae. Site of transition to urethra not determined.
Submitters
Contributors: Keast Lab
Submitted By: Keast Lab
Batch ID: 579
Submission ID: 22385
Expression Mapping

Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
   
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
   
Nerve Density:

Relative to Total:
High
Medium
Low
   
Relative to P0/Adult:
Increase, large
Increase, small
Decrease, large
Decrease, small
   
Contains note
View annotated components as a list Show annotation under groups information
Antibody
Protein:
Symbol: ACTA2
Name: actin, alpha 2, smooth muscle, aorta (NCBI ID: 59)
Name: anti-Actin, alpha-Smooth Muscle[clone 1A4]
Accession ID: No MGI ID (maprobe:9039)
Epitope (Uniprot ID): unknown
   
Supplier: Sigma
Catalogue Number: A5228
Lot Number:
   
Type: Monoclonal
Host: Hybridoma 1A4
Purification Method: purified immunoglobulin
   
Immunoglobulin Isotype: IgG2a
Variant Detected:
Species Specificity: mouse, rabbit, human, rat, goat, guinea pig, other species
Curator Notes:
Detection System:
Direct Label:
Secondary Antibody: Alexa Fluor 647 donkey anti-mouse (Mol Probes)
Signal Detection Method: Alexa Fluor 647
Detection Notes: Secondary antibody dilution 1:500
   
Dilution:
Notes:
Lab Antibody ID:
Specimen
Stage: Fetal 18wk
Tissue: bladder,ureter
Strain:
Genotype:
Sex: male
Specimen Preparation:
section
Fixation Method: 4% paraformaldehyde
Embedding: Cryosection
Experiment Notes:
De-identified tissue was obtained following informed consent.

 

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