Expression Database

GUDMAP:22377

Gene:	-
Stage:	Theiler Stage 28
Tissue:	dorsal root ganglion
Species:	Mus musculus
Assay Type:	IHC section

Images

	Red channel (DyLight 594) shows staining for Isolectin GS-IB4; green channel shows Htr3a-EGFP expression
	Green channel shows Htr3a-EGFP expression
	Red channel (DyLight 594) shows staining for Isolectin GS-IB4

Results Notes

Expression of Htr3a-EGFP transgene and staining for Lectin GS-IB4 is observed in dorsal root ganglion (EMAPA:16668). EGFP expression is present, strong, and visible in single neuronal cell bodies of the ganglion.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	Dennis P. Buehler
Submitted By:	Dennis P Buehler, Vanderbilt University, Nashville, TN, USA, dennis.p.buehler@vanderbilt.edu
Batch ID:	557
Submission ID:	22377

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: -

Name: ()

Name:

DyLight 594 Labeled GSL I - isolectin B4

Accession ID:

No MGI ID (maprobe:9036)

Epitope (Uniprot ID):

unknown

Supplier:

Vector Labs

Catalogue Number:

DL-1207

Lot Number:

Type:

Polyclonal

Host:

Purification Method:

Immunoglobulin Isotype:

Variant Detected:

Species Specificity:

Griffonia simplicifolia

Curator Notes:

Isolated from Griffonia simplicifolia. Sugar Specificity: Galactose. GSL I-B4 isolectin contains only the B subunits.

Detection System:

Direct Label: DyLight 594 conjugate

Secondary Antibody:

Signal Detection Method:

Detection Notes:

Dilution:

1:200

Notes:

DyLight 594 Labeled GSL I - isolectin B4 (Vector Labs, Cat#DL-1207). Antibody stored at 4oC. Diluted 1:200 in block with 10% normal donkey serum; applied to cryosections on slides (~500uL per slide) and incubated in a humidified chamber overnight at 4oC then rinsed liberally with 1X PBS (sterile filtered).

Lab Antibody ID:

IB4-DyLight594

Specimen

Stage:

TS28

Other Staging System:

P 90

Tissue:

dorsal root ganglion

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type

Sex:

female

Specimen Preparation:

section

	Fixation Method:	Neutral buffered formalin
	Embedding:	Cryosection

Experiment Notes:

Camera settings for images 1, 2 & 3: LSM 510 Meta Confocal Microscope; Objective: Plan-Apochromat 20x/0.75; Laser Power for Image 1: 488nm-25%, 543nm-15%; Laser Power for Image 2: 488nm-25%; Laser Power for Image 3: 543nm-15%; Filters: BP 505-550 (EGFP), BP 585-615(DyLight 594); Pinhole 140um; Tiled image.

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:22378	same section