Expression Database

Tissue Summaries

Expression Database

GUDMAP:21027

Gene:	Acta2, actin, alpha 2, smooth muscle, aorta
Stage:	Theiler Stage 21
Tissue:	primitive bladder
Species:	Mus musculus
Assay Type:	IHC section

Images

	Montage. Inverted Greyscale.
	Montage. Inverted Greyscale.
	Montage. Inverted Greyscale.
	Montage. Inverted Greyscale.

Submitters

Principal Investigator(s):	Janet Keast, University of Melbourne, Melbourne, Australia, janet.keast@unimelb.edu.au
Contributors:	Janet Keast, Elsbeth Richardson, Adam Wallace
Submitted By:	Adam Wallace, University of Melbourne, Melbourne, Australia, a.wallace@unimelb.edu.au
Batch ID:	370
Submission ID:	JK_31012013_025

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: Acta2

Name: actin, alpha 2, smooth muscle, aorta (MGI:87909)

Name:

anti-alpha SMA

Accession ID:

MGI:5497006 (maprobe:8754)

Epitope (Uniprot ID):

P62736

Supplier:

Abcam

Catalogue Number:

ab5694

Lot Number:

Type:

Polyclonal

Host:

rabbit

Purification Method:

affinity purified

Immunoglobulin Isotype:

IgG

Variant Detected:

Species Specificity:

mouse, rat, chicken, guinea pig, cow, dog, human, pig

Detection System:

Direct Label:

Secondary Antibody: Donkey anti-rabbit (Jackson Immuno Research Laboratories Inc #711-001-003)

Signal Detection Method: FITC

Detection Notes: Secondary Antibody Dilution: 1:1000

Dilution:

1:5000

Notes:

Lab Antibody ID:

Specimen

Stage:

Other Staging System:

13.5 dpc

Tissue:

primitive bladder

Strain:

C57BL/6

Genotype:

wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Cryosection

Experiment Notes:


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