GUDMAP Protocols
Editorial Office
- ISH / IHC / Tg Data
- Standards for in GUDMAP in situ hybridisation and immunohistochemistry entries
- Manual checking protocols
- Automated checking protocols
- Annotation Tool checking protocols
- Microarray Data
- Standards for GUDMAP cDNA microarray entries
- SOFT mapping protocol
- Normalisation of cDNA microarray CHP files
- Manual checking protocols
- Database Development
- Quality Control
- Testing
- Bug Fixing
- Data Submission Flow Diagram
Capel Group
- FACS Protocol
- RNA extraction and sample preparation for Affymetrix Gene 1.0 ST arrays
Gaido Group
- Fluorescent Immunocytochemistry Protocol on Frozen sections
- Whole-Mount Indirect Fluorescent Immunohistochemistry – Double or multiple staining
- Staining of frozen sections for LCM
- RNA isolation from LCM samples
- RNA isolation from whole testis
- RNA amplification and labeling of LCM samples for microarrays
- Microarrays
- GUDMAP - SOP "In Situ-Hybridization"
- GUDMAP - SOP "Fluorescent In Situ-Hybridization"
- GUDMAP - SOP "One-color FISH Protocol"
- GUDMAP - SOP "Two-color FISH Protocol"
- Riboprobe Synthesis
- Determination of Cellular Localization of ISH Patterns
Lessard Group
- Tissue isolation and embedding in OCT (Optimal Cutting Temperature Compound)
- Cryostat Sectioning
- Laser Capture Microdissection (Adapted from the Arcturus protocols)
- RNA Isolation
- RNA Amplification
- Microarray Screening
Little Group
- Digoxigenin-labelled Riboprobe Synthesis from a PCR-generated DNA template
- Semi-Automated Section in situ Hybridisation for 7µm paraffin sections
- Semi-Automated Whole Mount in situ Hybridisation
- Immunohistochemistry on paraffin sections
McMahon Group
- Annotation Guidelines for In Situ Hybridization
- Digoxigenin-Labeled In Situ Hybridization for E15.5 Wholemount Kidneys
- Digoxigenin-Labeled In Situ Hybridization for P1 Mouse Kidney Sections
- Optimized Vibratome Section in situ Hybridization for P1 Mouse Kidney Sections
- Riboprobe Synthesis for In Situ Hybridization
- HYB Solution Guide
Potter Group
- Preparing OCT blocks of kidney tissue
- Sectioning
- Processing slides for LCM
- Laser Capture Microdissection
- LCM RNA preparation
- FACS analysis for E15.5 mouse kidneys
- Target Amplification
Southard-Smith Group
- Isolation of YFP+ neural crest derivatives from embryonic tissues
- RNA isolation procedure from flow sorted cells
Vezina Group
- Immunohistochemistry (IHC) for in-situ hybrdisation (ISH)-stained mouse sections
- Digoxigenin-11-UTP-labelled riboprobe synthesis
- Sectioning of paraformaldehyde-fixed mouse urogenital sinus (UGS) tissues with a vibrating microtome
- In situ hybridisation on vibrating microtome-cut mouse tissue sections
