GUDMAP

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Gaido Group Protocols

  • Fluorescent Immunocytochemistry Protocol on Frozen sections
  • Whole-Mount Indirect Fluorescent Immunohistochemistry – Double or multiple staining
  • Staining of frozen sections for LCM
  • RNA isolation from LCM samples
  • RNA isolation from whole testis
  • RNA amplification and labeling of LCM samples for microarrays
  • Microarrays
  • GUDMAP - SOP "In Situ-Hybridization" (html:page1|page2|page3)(pdf)
  • GUDMAP - SOP "Fluorescent In Situ-Hybridization" (html:page1|page2|page3)(pdf)
  • GUDMAP - SOP "One-color FISH Protocol"
  • GUDMAP - SOP "Two-color FISH Protocol"
  • Riboprobe Synthesis (html)(pdf)
  • Determination of Cellular Localization of ISH Patterns

 

Group Description


 

Sponsoring Agencies:

  • Department of Health and Human Services
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • The National Institute of Child Health and Human Development

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