Gaido Group

  • Fluorescent Immunocytochemistry Protocol on Frozen sections
  • Whole-Mount Indirect Fluorescent Immunohistochemistry – Double or multiple staining
  • Staining of frozen sections for LCM
  • RNA isolation from LCM samples
  • RNA isolation from whole testis
  • RNA amplification and labeling of LCM samples for microarrays
  • Microarrays
  • GUDMAP - SOP "In Situ-Hybridization"
  • GUDMAP - SOP "Fluorescent In Situ-Hybridization"
  • Riboprobe Synthesis
  • Determination of Cellular Localization of ISH Patterns

Lessard Group

  • Tissue isolation and embedding in OCT (Optimal Cutting Temperature Compound)
  • Cryostat Sectioning
  • Laser Capture Microdissection (Adapted from the Arcturus protocols)
  • RNA Isolation
  • RNA Amplification
  • Microarray Screening

Little Group

  • Digoxigenin-labelled Riboprobe Synthesis from a PCR-generated DNA template
  • Semi-Automated Section in situ Hybridisation for 7µm paraffin sections
  • Semi-Automated Whole Mount in situ Hybridisation
  • Immunohistochemistry on paraffin sections

McMahon Group

  • Annotation Guidelines for In Situ Hybridization
  • Digoxigenin-Labeled In Situ Hybridization for E15.5 Wholemount Kidneys
  • Digoxigenin-Labeled In Situ Hybridization for P1 Mouse Kidney Sections
  • Optimized Vibratome Section in situ Hybridization for P1 Mouse Kidney Sections
  • Riboprobe Synthesis for In Situ Hybridization
  • HYB Solution Guide

Potter Group

  • Preparing OCT blocks of kidney tissue
  • Sectioning
  • Processing slides for LCM
  • Laser Capture Microdissection
  • LCM RNA preparation
  • Target Amplification