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ЏW series info Ў ; 5 ; G С С ai ќ Z' Platform Distribution Array Manufacturer protocol Tissue/Cell type Sample molecule Sample Label Title Summary Overall design Type Sample ID table Standard Fields table8 Gene expression profiles of mouse postnatal day 1 ureter Ureters were dissected away from the kidney and bladder and snap frozen in liquid nitrogen. Samples were briefly stored at -80C until total RNA was isolated. Sample ID Description Variable Table Variable Samples Replicates table! The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital tract. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of postnatal day 1 ureters.< Gene expression analysis of the postnatal day 1 mouse ureter 51U_MOE430_2 postnatal day 1 ureter* 49U_MOE430_2 | 50U_MOE430_2 | 51U_MOE430_2 Ureters were isolated from postnatal day 1 SMGA/EGFP transgenic mice and RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip. day 1 mouse/Potter/ day 1 mouse/Potter; this is the reference data. Male and female postnatal day 1/whole mouse mouse day 1 reference none postnatal day 1 total RNA ND in situ oligonucleotide
commercial
Affymetrix Affymetrix standard protocol Qiagen: RNeasy
Mouse430_2 Epicentre Potter protocols 10 ug Biotin Affymetrix standard protocol 3 Affymetrix GCOS and Gene Spring and Avadis programss The long term goal is to generate a gene expression atlas of the developing and mature mouse genitourinary system.
NCBI Taxonomy ID Platform Technology Replicate type Unique ID Reference Table Technical Reference Corresponding Biological Sample Submitted_By Submitted_Institution day1ureter_assoc_JCSV This sample represents the universal standard for cross comparison of microarray data. Submitted_Country Submitted_Telephone Submitted_Email PI_Name PI_Institution PI_Mailing_Address PI_City PI_Zip_Code
PI_Country PI_Telephone PI_Email Sample name
Ontology name Genus / Species Strain Mutations
Pooled sample Pool size Sex Developmental stage Dissection method Sample image A260/280 Bioanalyzer RIN Array Manufacturer
Array Name RNA extraction protocol Target Amplification protocol 49U_MOE430_2 50U_MOE430_2 MSREF_MOE430_2 49U 50U 51U MSREF ureter Submitted_Mailing_Address Submitted_City Submitted_Zip_Code Associated File Biological replicate Technical replicate Mouse day 1 reference Tissue type whole mouse) Amount labeled target hybridized to array Array Hyb/Wash/Scan protocol Data analysis method Reference used Experimental Design Experimental goals
Theiler Stage Somite count Rounds of amplifcation% Target Amplification manufacturer/kit GCOS Tgt Value Developmental landmarks John C. Szucsik2 Cincinnati Children's Hospital Research Foundation$ TCHRF 3049 3333 Burnet Ave. Cincinnati
45229-3039 USA 513-636-8312 john.szucsik@cchmc.org James L. Lessard TCHRF 3047 3333 Burnet Ave.
Cincinnati 513-636-8308 james.lessard@cchmc.org Mus musculus FVB/N SMGA/EGFP transgenic no N/A yes; 4 males and 4 females none; whole animals used NA4 whole ureters dissected away from kidney and bladderџ Ђ
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